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The kcat values of the mutants were in the range of 47.2-110 47.2-110s−1values were 52-120% of theseild-type enzyme values
All mutants were in the Col-0 background except for hen1-1 whish in in the La-er background.
These data suggest that phenotypic changes observed in the mutants were in large part mediated by p53.
Indeed, the decrease oligomer amount for the S7/A and S8/A mutants were in agreement with the absence of fibrilization or their longer lag phases of fibrilization.
PCR fragments containing T7 Promoter sequences or linearized pUC19 plasmid containing various Vpr mutants were in vitro-transcribed using a mMessage mMachine T7 Ultra kit (Ambion) according to the manufacturer's recommendation.
All the mutants were in the s288c strain background.
Similar(41)
The mutants are in two classes, within dimers and between dimers.
These mutants are in the Arabidopsis thaliana Columbia accession (Col).
All mutants are in the Arabidopsis Columbia ecotypic background (Col-0).
Both mutants are in Col-0 background.
All mutants are in Col-0 background.
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