Homozygous T-DNA insertion mutants were identified by PCR-based genotyping.
To identify the molecular machinery that mediates mechanotransduction in hair cells, zebrafish mutants were identified with defects in balance and hearing.
C. difficile transconjugants were selected on BHIS plates supplemented with D-cycloserine, kanamycin, and thiamphenicol and potential TargeTron mutants were identified by plating on erythromycin.
From a small panel of putative inhibitors, compounds that potently and selectively target the inhibitor-sensitized PTPH1 mutants were identified.
Using high throughput screening, more than 20 YqhD mutants were identified to show activity on NADH as a cofactor.
Replacements of amino acid on beneficial mutants were identified as T66L/D70N, T66V/D70N, E83K, E83H and E83N.
Four unique mutants were identified that maintain the ability to form viral particles, with one showing improved transduction on both 293T and BEAS-2B cells.
Recently, several monogenic root-specific mutants were identified in rice (Table 1).
The equivalent mutants were identified manually, and the execution and analysis were performed automatically by scripts.
Lower: 2 mutants were identified in 96 independent lines transformed the target-1 sgRNA:hSpCas9.
Upper: 14 mutants were identified in 90 independent lines transformed the target-1 sgRNA:pSpCas9.
