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MZ yap1 −/− mutants were, however, still lower than double mutants (−27%, p = 1.09E-11).
The 103+/ kasA mutants were, however, found significantly more frequently in phagolysosomes and this effect could be reversed by expressing the recombinant wild-type kasA gene in the mutant (Fig. 6A).
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The phenotypes of all three mutants are, however, stable for at least 6 months.
(F ) Out of plane crossing of C4da neuron dendrites in Ret mutants was however highly elevated (mean ± SD, n = 8; p < 0.001, Student's two-tailed t -test).
The increase observed in the mutant was however significantly less than that elicited by the wildtype hOct4 promoter sequence, leading us to conclude that the trans-activation potential of the human Oct4 promoter by B-MYB is elicited, at least in part, through the MYB-binding site located in the proximal promoter of this gene.
This mutant is, however, affected rather in secretion with endocytosis being more or less intact [ 36].
The phosphorylation-deficient receptor mutant was, however, seen to be robustly coupled to Gq/11-protein pathways such as phosphoinositide hydrolysis and calcium signalling.
The period of the double mutant was however intermediate between the single mutants, being slightly reduced compared to the soc1 single mutant (τ = 24.49 ± 0.24 h vs. 24.98 ± 0.22 h, P= 0.048).
Current amplitudes of the double mutant M132T/N143S were however similar to the M132T mutant, but did not show a larger increase in current amplitude than the M132T mutant alone (Figure 3).
All four models generated a double map for the Isl2-EphA3ki/ki muthere there were, however, subtle differences between the model results and experimental data.
All the mutants were active; however, major changes in the kcat values were measured (15- to 500-fold decrease).
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