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As expected, the mutants were generally depleted compared with the wild type.
However, the levels of mRNA accumulation in these mutants were generally lower than those observed in the mmi1-ts3 mutant.
Similar to wild-type plants, the roots of cad2-1 mutants were generally more stressed than the leaves.
In contrast, E650 mutants were generally insensitive to strontium activation, and the mutant displaying the highest sensitivity was again E650R, in keeping with our findings regarding calcium activation.
The yields of expression obtained with all of these mutants were generally somewhat lower than the yield of wild-type CYP3A4.
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However, recA mutants are generally defective and grow poorly compared to the wild type [23].
Ectopic bristles in the loss-of-function mutants are generally observed close to extant ones, suggesting that they arise from the proneural clusters of ac-sc expression.
Since transmission fitness of compensated mutants is generally lower than that of the wild-type virus, the spread of disease is reduced by increasing drug use to moderate levels (Figure 3a, solid curves).
We did notice, however, that levels of TFIIB occupancy in activator mutants was generally higher than the occupancy of Pol II, which may be an artifact of the ChIP assay (i.e. TFIIB cross-links more efficiently), or may represent a more stable association at the promoter, even in the absence of activator.
Despite the reversed polarity, lin-44(n1792) mutants are generally healthy and do not display severe morphological or physiological defects.
These mutants are generally dwarfed and exhibit rounded, dark green leaves, delayed flowering, reduced male fertility and seed set, and delayed senescence.
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