Sentence examples for mutants were first from inspiring English sources
The eight mutants were first equilibrated to avoid deformations of the secondary and tertiary structure and to minimise alterations in the functionality of the chimera enzymes that were obtained.
Mutants were first purified through a Ni-NTA column followed by a DEAE cellulose (Sigma-Aldrich) column (2.5 × 10 cm) using a BioLog FPLC System (Bio-Rad Laboratories).
The mutants were first tested for their sensitivity to RAL in a 3'end processing assay.
In a reverse experiment ARF6 mutants were first precipitated and the presence of GFP-A-RAF in the precipitate was demonstrated by immunoblotting with anti-GFP antibodies.
All homozygous mutants were first generation homozygotes.
Dauer larvae of these mutants were first preconditioned, and then further desiccated at 60% relative humidity.
We thus find that morphological defects in ablated mutants are first seen at early somitic stages.
The biological activity of these mutants was first tested in a RNP replication system using a CAT gene-containing viral replicon.
Non-transgenic homozygous gpr124 mutants are first distinguishable from wild-type siblings by their reduced growth rate and eventually by the disrupted pigmentation patterns of their skin after metamorphosis (after 14 dpf).
Viability of s288c strains expressing SCC4 mutants was first confirmed by complementation of pGAL1- SCC4 repression as shown for Figure 2 figure supplement 3 using plasmids bearing the SCC4 chromosomal locus.
Since spreading cell death in older atg mutants is first macroscopically visible several days post infection, and as NPR1 has previously been implicated in restricting HR cell death triggered by RPM1,, we investigated the importance of NPR1-dependent HR cell death.
