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Due to the background of the concomitant chemical reaction, the low activities of the mutants were not exactly quantifiable.
The simulation setups were exactly the same for the WT and mutant complex.
The procedures were exactly the same as described previously except the shotgun library clones were constructed with the nfi- mutant of E. coli DH5α as host bacterium.
eNo results were exactly zero.
Hybridisations are performed under conditions in which fluorescently tagged target DNA only binds to wild-type and mutant oligonucleotides that are exactly complementary to its sequence, leading to a gain-of-hybridisation signal at selected oligonucleotide positions.
Unfortunately, most cell envelope mutants were obtained by treatment of recipients with random mutagens [ 17, 18] so the mutated sites could not be exactly determined.
Another advantage of the project is that a large number of mutants are studied in exactly the same way – in the same environment, by the same people.
But a tiny fraction of the new mutants were super-mutants: their owners fared well in both situations.
Other mutants were generated similarly.
Strikingly, the chances of generating a parasite as a HD = 1 mutant of the master sequence are exactly zero (0.0005 at HD = 2), despite the fact that a single mutation disrupting the catalytic structure of a unit of replication could suffice.
Note that the restoration of glycosylated synaptomatrix composition in dGALT nulls was exactly opposite for dGALK-null and sgl-overexpression double-mutant conditions (Figs 6 and 8).
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