Sentence examples for mutants were established from inspiring English sources

Protocols for iterative rapid mutation of S. mobaraensis with helium-driven ARTP system and effective tube screening method of the mutants were established.

ZHBTc4 ES cell lines stably expressing Oct-4 truncation mutants were established as described previously [ 26].

Rest GT D047E11) (Rest GT) mutants were established by blastocyst injection of the D047E11 GT clone (GenBank Acc.: DU821609).

After exposing cells to either EMS or X-rays, clonal lines of potential mutants were established by isolating individual cells through limiting dilution (i.e., on average, plating less than one cell/well) into 96-well plates containing rosette-inducing A. machipongonensis conditioned media (ACM; Figure 2).

However, initial screening of the mutants was established on the index ratio of the zone of clearance.

Analysis of the simulated CG trajectories reveals a straightforward molecular mechanism by which the multispanning topology of EmrE and its mutants is established.

We therefore expect that the collection of moss mutants being established here will help to identify novel genes previously unknown in plants [ 7, 12] and will allow to rapidly link DNA sequence and functional information.

For that reason, the effect of peptides on CaM mutants was established before examining the Ca2+ dissociation kinetics of wild-type CaM, to identify the rate that corresponds to each lobe's action.

Clones stably expressing either wild type RhoA or the RhoA-F mutant were established.

Importantly, two independent clones expressing each G9a mutant were established in order to control for potential clonal artifacts.

Once the stability of the mutant was established, the above steps were repeated with more stringent selection criteria in form of 30/40 % walseth cellulose and higher concentration of glucose/glycerol.