Sentence examples for mutants were engineered from inspiring English sources

VP1 mutants were engineered and characterized for RNA synthetic capacity in vitro.

To confirm the model, double and quadruple cysteine LexA mutants were engineered.

Using sequence- and structure-based approaches, 48 CLF multiple mutants were engineered and analyzed.

To investigate the cellular and molecular functions that may be altered by these mutations, CEP290 mutants were engineered from a mouse wildtype Cep290 N-terminally EGFP- or mCherry-tagged construct.

The low-fidelity mutants were engineered by knocking-in DNA sequences that direct changes of leucine 2618 to either phenylalanine (L2618F) or methionine (L2618M) of Pol ζ.

Tobacco mosaic virus mutants were engineered to alter either the stability or surface chemistry of the virion: within the coat protein, glutamic acid was exchanged for glutamine in a buried portion to enhance the inter-subunit binding stability (E50Q), or a hexahistidine tract was fused to the surface-exposed carboxy terminus of the coat protein (6xHis).

The pEGFP-SHP2 WT and C/S mutant were engineered by inserting a coding region into the SalI and BamHI sites of pEGFP vector (Stratagene).

To investigate this possibility, three additional IsdBN1 protein constructs (i.e., IsdBN1 F164D and Y167D single mutants, and F164D/Y167D double mutant) were engineered, with F164 and Y167 replaced by aspartic acid.

Escherichia coli BL21 DE3)/ΔpgiΔzwfΔgalU mutant was engineered by overexpressing thymidine diphosphate (dTDP -d-glucose synthase (tgs), dTDP -d-glucose4,6-dTDP -d-glucose), and a synthaseinotgsnsferase (wecE) from different sources to produce a pool of dTDP-d-glucose,6-dhdeoxy-d-gandctose in the cell cytosol.

This Pfu DNA polymerase mutant was engineered to overcome uracil stalling.

The Ffh(E153C) single-cysteine mutant was engineered using the QuikChange mutagenesis kit (Agilent, Santa Clara, CA).