Sentence examples for mutants were done from inspiring English sources
Expression and purification of wild-type AfUGM and all mutants were done under conditions previously reported.
Expression, purification, and spin labeling of the mutants were done as described in the Supporting Information.
The genotyping and selection of homozygous triple mutants were done similarly, using the primers listed in supplementary material Table S1.
Secretory mammalian expression in HEK293T cells and purification via affinity chromatography of EGb-Ki4 scFv) EGb-Ki4 scFvnts were done anddescritsd previously.
Identification of the positive transformants that were homozygous for the T-DNA insertions resulting in the irx9-2, irx7, and irx8-6 mutants were done as described by [ 33].
Here, we must note that the measurements for the cathodic peaks of both mutants were done by a mathematical deconvolution (detailed explanation appears in the Experimental Section) due to reductive catalytic peaks that appeared in more negative potentials probably due to bacterial lysis on the surface.
Expansion of Cer1 and Foxa2 in Itga5 mutants was done on a 129S4 background.
Analysis of HO-3 or C215 binding to native EpCAM mutants was done using HEK293 cells stably or transiently transfected with the above mentioned expression vectors.
Screening of photosynthetic and pigment deficient mutants was done by visual inspection and monitoring of growth under different light intensities in heterotrophic, mixotrophic and photo-autotrophic conditions.
Test labeling of the two double mutants was done with two rhodamine isomers, 5-IATR and 6-IATR (5- and 6-iodoacetamidotetramethylrhodamine), and the fluorescence response to ADP binding was measured.
The initial screen of 163 TR mutants was done in 36-well plates containing YP-dextrose (YPD) or YP-glycerol (YPG), since glycerol is only a substrate for mitochondrial oxidation.
