Sentence examples for mutants were digested from inspiring English sources
To locate the disrupted region containing the MiniHimar RB1, the chromosomal DNAs of these mutants were digested by BamH1, followed by self-ligation and sequencing [39].
Genomic DNAs from RFM mutants were digested with HpaII or MspI (New England Biolabs), electrophoresed on 1% agarose gels, and transferred to Genescreen nylon membrane (NEN, Boston, MA).
Plasmids containing the desired RT mutants were digested with AgeI and BclI (New England Biolabs) and the respective fragments were inserted into the pTN7-Stopp HIV-1 expression plasmid using the same restriction sites.
Plasmids containing NBD1/linker Cys mutants were digested with SpeI and AgeI and gel-purified.
SSA-positive PCR products from spn-A mutants were digested by SacI and 100% cleaved, indicating conversion of the DSB site to the wild-type SacI recognition sequence (n = 127).
To generate each pCB1531 AVR-Pik pCB1531 AVR-PikikD mutant (H46E, I49E, R64A, D66R, A67D, promoter -AVR-PikDC57:AVR-promoter -AVR-PikDdigested with XbaI and EcoRI, and inserts were exchanged to the mCherry gene at the same sites of promoter -AVR-PikDer)-AVR-Pik-mutanty (SH46Ea et al., 2013).
In inverse splinkerette PCR, genomic DNA from insertion mutants was digested with a restriction enzyme that cuts once (or very few times) in the insertion vector and frequently in the genome (e.g. EcoR V in this work).
Finally, to examine DRM2-dependent methylation at the transposable element AtSN1, DNA from both wild type and jmj14 mutants was digested with the restriction endonuclease HaeIII that cleaves GGCC sequences, but not GGmCC.
For Southern analysis DNAs of the wt and the Afeng2 mutant were digested with Xba I and Eco RV and hybridized with a 459 bp fragment of the upstream flanking region of ENG2.
Ten µg genomic DNA of the transposon mutant was digested with 40 U PstI overnight at 37°C in 40 µl restriction buffer, purified by phenol/chloroform extraction, and the pellet was resuspended in 25 µl TE buffer.
50 ng genomic DNA from each DH5α Kmr mutant was digested with Csp6I endonuclease.
