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The relative activities of all PtmU4 mutants were determined using a 5-CoA concentration of 500 μM.
In each set of experiments, the deamination activities of mutants were determined in relation to wt hAID.
Some of the mutants were determined as superiors for the studied variables.
In separate experiments, the pharmacological profiles of [3H]DA uptake in PC12 cells transfected with WT hDAT or these mutants were determined.
Binding changes of the individual mutants were determined using three saliva samples that are Lea (Nonsec/Lea), Ley (Sec/Ley) and Leb (Sec/Leb) positive, respectively (Fig. 8B).
The metal contents of the wild-type enzyme and mutants were determined by inductively coupled plasma optical emission spectrometer (ICP-OES), PerkinElmer 5300DV.
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Statistical difference in soma size between the RHEB WT and mutants was determined using one-way analysis of variance (ANOVA) followed by Tukey's post hoc test for multiple comparisons.
However, in order to confirm that the low gluconic acid production was not caused by different growth rates of the Δgox mutants, the biomass of the Δgox mutants was determined after the growth experiment.
The feeding ability of itpr mutants was determined quantitatively by measuring ingestion of colored food (Figure 2).
The degree of cytotoxicity among the 28 mutants was determined by measuring the release of lactate dehydrogenase (LDH), a cytoplasmic protein commonly used to identify cell lysis.
The photosynthetic performance of the site-directed mutants was determined by chlorophyll fluorescence analyses, using the Photo II device (Biosensor Srl, Palombara Sabina, RM, Italy, http://www.biosensor.it/).
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