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A series of STING mutants were constructed as shown in Fig. 3C.
Affinity mutants were constructed with binding constants ranging from 50 μM to 1 mM.
In addition, two more mutants were constructed by splicing of the C-terminal non-conserved sequence.
On the basis of the predictions from this algorithm, mutants were constructed and characterized for a model protein, thioredoxin.
Fourteen mutants were constructed by the insertion of one N-glycosylation consensus sequence into different positions of the cytokine.
Seven different mutants were constructed harboring, separately or in combination, three different genetic modifications to Synechocystis' metabolic network.
Four representative IN mutants were constructed in the context of the infectious NL4.3 HIV-1 viral clone.
Single and double bat1Δ and bat2Δ mutants were constructed.
Mutants were constructed using the primers in Table S1.
Insertion mutants were constructed using the suicide plasmid pVO155 [36].
Rabbit and mouse wild-type CRT and site-directed mutants were constructed in pBAD vector.
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clones were constructed
mutants were affected
mutants were named
mutants were indicated
mutants were expressed
mutants were designed
mutants were screened
mutants were solved
mutants were confirmed
mutants were recovered
mutants were obtained
mutants were discovered
mutants were set
mutants were generated
mutants were monitored
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