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Directed mutagenesis also supported the structural model constructed on CAL-A template: the functional properties of various mutants were consistent with their structure-based putative involvement in the oxyanion hole, substrate specificity, acyltransfer or hydrolysis catalysis and structural stability.
These data on AGP quantification in the mutants were consistent with the expression profile data for GALT2-GALT6.
The kinetics of the mutants were consistent with their predicted structural features obtained by modeling, and confirmed the importance of position 441 for BAL specificity.
Together, results obtained in the analysis of dcl, rdr, and pol mutants were consistent with the interpretation that the 24-nt small RNA under study is produced by the PolIV/RDR2/DCL3 pathway, a typical feature of hc-siRNAs.
Similar(56)
Finally, using cell lines engineered to express the TRIM5αhu gene product, recently identified as the Ref1 restriction factor, and RNAi technology to knock-down expression of TRIM5αhu in human cells, we directly demonstrate that the unique patterns of restriction observed in human cells with the different mutants are consistent with a TRIM5αhu-mediated restriction.
The following shapes mutants are consistent with this surface tension argument.
The electrophysiological phenotype we observed in wnt2 mutants is consistent with both the syb and brp mutant phenotypes.
The enhanced tolerance seen in co mutants is consistent with the finding that 35S::LOV1 plants are tolerant to cold temperature.
The phenotype of mutant embryos and the pattern of gene expression in mutants are consistent with a role of mp66α in gene silencing.
Our results showing that centromere coupling is abolished at all time points in zip1 deletion mutants are consistent with this sort of direct structural role for Zip1.
The fact that the pyoverdine receptor FpvA, the major uptake system for iron in P. aeruginosa [48], [56], is amongst these mutants, is consistent with this interpretation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com