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During 2002 2008, 93% and 84% of mutants were collected for subtypes N1 and N2, respectively, in the NCBI group.
Cell lysates of lines transfected with PrPC and its mutants were collected, and PrPC glycosylation and processing were assessed before and after treatment with PNGase.
Images of GFP-tagged phosphorylation site mutants were collected as a series of 13 optical sections, with a step size of 0.3 µm.
For superoxide detection, the hyphae of the wild-type strain Guy11 and ΔMovam7 mutants were collected from 3-day-old CM agar plates and stained with 0.6 mM nitroblue tetrazolium (NBT) aqueous solution for 2 hours after which the reaction was stopped by adding ethanol.
The two-dimensional 1H-15N Hspectractra, performed to monitor the folding of the protein mutants, were collected on 100 µM samples of 15N-labeled E,E- and E,Zn-hSOD1SS mutant proteins in 20 mM sodium phosphate buffer (pH 7).
CD spectra of purified wild-type AcrB and its mutants were collected as described previously.
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Data for the C425S mutant were collected to 2.4 Å resolution on beamline BL-17A of the Photon Factory in Japan using an ADSC Q270 detector.
To this end, G1-phase daughter cells from the wild-type and quadruple sap mutant were collected by centrifugal elutriation and cells were allowed to progress through the cell cycle.
Stems of WT and xylp7 mutant were collected respectively, including stems of 60, 70, 80, and 90 days-old plants.
Polysome microarray data from the rpl24b mutant and the pab2 pab8 mutant were collected using sucrose gradient fractionation of ribonucleoprotein complexes from Arabidopsis seedlings and were processed as described in Methods.
According to the manufacturer's recommendation, mutant strains were selected and transferred onto Luria Bertani agar (10 g/L Bacto Tryptone, 5 g/L Bacto Yeast Extract, 10 g/L NaCl and 15 g/L Bacto Agar) containing 30 μg/mL kanamycin, and the resulting tnaA-deficient mutant was collected and used as a host for the subsequent experiments.
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changes were collected
transformants were collected
engineers were collected
mutants were cultivated
mutants were described
mutants were confirmed
mutants were detected
mutants were obtained
mutants were recovered
mutants were discovered
mutants were generated
mutants were set
mutants were monitored
mutants were indicated
mutants were named
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