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Moreover, ∆makCB, ∆makDCB, ∆makBA, and ∆makDCBA mutants were clearly attenuated (Supplementary Fig. 1B).
In contrast, the protein levels of the C-terminal deletion mutants were clearly increased when the proteasome was inhibited, indicating poly-ubiquitination (Figure 4D), therefore the most C-terminal region is required for USP25 estabilization.
The levels of P110 and P140 detected in the C30 mutants were clearly higher than those observed previously for T192 suggesting that additional factors are needed to explain the dramatic reduction of the levels of these proteins in the T192 mutant.
Among the 27 waxy mutant lines of maize, including 11 EMS-induced, two of the EMS-induced mutants were clearly intermediate.
In the case of P. irregular, however, host resistance correlated with high ABA levels, whereas ABA mutants were clearly more susceptible.
Our mutants were clearly resistant to age-related obesity, notably exhibiting elevated insulin sensitivity, which reminds the metabolic features of some long-lived mutant mouse strains (Bartke & Westbrook, 2012).
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For example, Rc5-1 and Rc5-7 treated sclerotia showed mycoparasite hyphae beginning to extend outwards, and by 16 h, mycoparasitism was evident on all sclerotia infected with transformants and the wild type strain, but the parasitic ability of mutants was clearly higher than the wild type (P < 0.05).
The enhancing effect of the mutants was clearly IKKβ-dependent, as tested again in IKKβ-deficient 3T3 cells.
Although higher than the mock, the degree of cell invasion in cells with N185D and S156A mutants was clearly less than that of cells with AQP5 (Fig. 2A, 2C).
We compared the viability of wild-type and mitochondrial mutant cultures 24 and 48 hr after reaching stationary phase by spotting cells in complex media plates (Figure 4A), and the results indicate that the elevated levels of ROS in these mutants are clearly deleterious for cell survival.
The pattern of expression of deletion mutants was clearly different in the absence and presence of p53.
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