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RT activities of mutants were calculated and compared to that of wild type pseudovirus.
After incubation at 37°C for 48 h the numbers of CFU were counted, and the frequencies of rifampicin resistant and streptomycin resistant mutants were calculated.
Extinction coefficients of the Cygb distal histidine mutants were calculated as previously described using HPLC.
Structural models of selected cancer-associated single-point mutants were calculated by the SWISS-MODEL server [ 37].
The GRAVY values for the double mutants were calculated for the obtained patches using Protparam Tool (Expasy) (Table 2).
KM values towards methanol for the parental strain and the CA2 and CA4 mutants were calculated as 0.62, 2.48, and 1.10 mM, respectively (Table 1).
Similar(53)
Relative ligand-binding capacity (represented by binding %) to different mutants was calculated by the MS response of the ligand released from a specific mutant divided by the MS response of the total ligand present in the incubation sample.
The wt TSHR was set at 100% and receptor expression of the mutants was calculated according to this.
The Cm value for ΔQ2, Q2R, and ΔP3 mutants was calculated to be 21.85, 19.84 and 17.94±0.05 M, respectively.
The concentration of the mature inhibin A or activin A mutants was calculated based on the immunoblot.
The level of wild-type TSHR was set as 100% and receptor expression of the mutants was calculated according to the level of wild-type TSHR.
More suggestions(15)
genes were calculated
transfers were calculated
clones were calculated
transformations were calculated
variants were calculated
mutants were confirmed
mutants were recovered
mutants were obtained
mutants were discovered
mutants were set
mutants were generated
mutants were monitored
mutants was calculated
mutants were named
mutants were indicated
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