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The acs-20 mutants and acs-22 mutants were backcrossed twice with N2.
Hemizygous mutants were backcrossed eight times with C57BL/6 mice to generate transgenic mice with a C57BL/6 genetic background.
With the exception of Ins3, which happened to be sterile, all Ins mutants were backcrossed with an rps15-1 strand and proved to be recessive.
The mutants were backcrossed four times with N2.
All mutants were backcrossed three to six times every few months.
The corresponding offspring mutants were backcrossed at least twice with the CBS999.97 1-2, wt) strains.
Similar(47)
In order to obtain isogenic strains, the mutants are backcrossed to the starting strain used for mutagenesis prior to sequencing to remove any unlinked mutations.
All transgenes and the dfoxo mutant were backcrossed at least six times into the wild-type outbred Dahomey population carrying the w 1118 mutation and cured of Wolbachia infection and frequently outcrossed back into the wild-type population.
The oslap6 mutant was backcrossed with the wild type (9311), and the resulting F1 plant was further selfed to generate the F2 population.
A confirmed homozygous T-DNA insertion mutant was backcrossed to Col-0 three times before allowing self-fertilization.
The sis7-1/nced3-4/sto1-4 mutwicewandbackcrossed thece and the sis7-2/nced3-5/sto1-5 mutant was backcronced once to wild-type Col before physiological assays were conducted.
More suggestions(15)
mutants were obtained
mutants were discovered
mutants were set
mutants were generated
mutants were monitored
mutants were indicated
mutants were affected
mutants were named
mutants were expressed
mutants were designed
mutants were solved
mutants were screened
mutants were compared
mutants were tested
mutants were analyzed
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