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T-DNA insertional mutants were available for each gene: line bet11-1, contanninsertionsertinn in exon 5 of BET11 (Fig. 1a) and bet12-1, contanninsertionsertinn in exon 2 of BET12 (Fig. 1a).
Since no insertional mutants were available in Arabidopsis, we obtained, via a RNAi strategy, plants in which dUTPase activity was partially decreased.
Many natural serine recombinases with distinct sequence specificities are known [14], and their catalytic domains could be co-opted to make ZFRs if suitable activated mutants were available [1].
Haploid deletion mutants were available from the deletion project consortium EUROSCARF.
Because no loss-of-function mutants were available in the L er ecotype, we downregulated ARF2 and ARF6 mRNA levels via artificial miRNAs (amiR).
If experimental data on the knockout and copy-number mutants were available, their signals could be compared with these predictions and thus used to validate the model or to identify its weaknesses.
Similar(52)
Since several GA biosynthesis and perception mutants are available in rice [22, 75], this question, as well as the function of OsSCR and OsSHR genes, should be addressed in the near future.
Such a silent exchange is recommended if no phenotypic screen of resulting mutants is available.
Another important feature is the extent to which known Arabidopsis mutants are available as a means of verifying the model.
Moreover, a comprehensive library of mutants is available [7] and powerful tools, such as RNAi, allow manipulation of gene expression.
This will enable systematic structure-function studies for genes, for which no mutants are available, or more importantly mutants that display highly pleiotropic phenotypes.
More suggestions(14)
transformants were available
postings were available
clones were available
variants were available
ones were available
mutants were recessive
mutants were stable
mutants were first
mutants became available
mutants were non-transformable
mutants were resistant
mutants were sucrose-sensitive
mutants were capable
mutations were available
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