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A total of 62 mutants were attenuated when checked individually, and 25 unique single transposon insertion mutations were identified from this group.
It also emphasized that although ORF75c− mutants were attenuated, they remained replication competent.
In contrast, septin mutants were attenuated in filament formation (Fig. 9A).
However, B-repeat mutants were attenuated even in Fg-deficient mice, implying that the B-repeats may have a second function, in addition to Fg-binding.
Because the B-repeat mutants were attenuated even in Fg-deficient mice, lack of Fg-binding is not sufficient to explain the properties of the mutants.
In support of this, ctpG mutants were attenuated for virulence in the guinea pig, supporting their role in this model [44].
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Furthermore, these SodA mutants are attenuated in their ability to cause death and to persist in vivo [4].
In order to determine whether any of the hypercytotoxic mutants are attenuated, BALB/c mice were infected with each of the 28 transposon mutant strains.
For example, knockout mutants of hlyX are unable to survive in lung epithelium, sequestered lungs or tonsils [29]; dmsA mutants are attenuated in acute disease [25]; and asp mutants cause less severe lung lesions than the wild type organism [24].
It has been reported that non-motile and non-autoagglutinating C. jejuni mutants are attenuated [37], and we hypothesized that these phenotypes may have changed during passage in mice.
When tested, PI 4,5 P2 binding for all mutants was attenuated relative to that of the wild type.
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mutants were diluted
mutants were confirmed
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mutants were obtained
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mutants showed attenuated
mutants were discovered
mutants were generated
mutants were set
mutants were monitored
mutants were named
mutants were indicated
mutants were affected
mutants were expressed
mutants were designed
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