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During the first vinification experiment (V1) the haploid and diploid pdc2Δ519 mutants were assayed against their respective BY4741 and BY4743 controls (Table 1).
The native form and cysteine/serine mutants were assayed for enzyme activity, free thiols, and the secondary structures by circular dichroism and Fourier transform infrared.
The resultant double mutants were assayed for their ability to grow on plates lacking histidine.
dop-3 single mutants were assayed off food for avoidance of dilute (30% and 10%) octanol.
The enzymatic activities of the wild type PDE9A2 and its mutants were assayed by using cAMP and cGMP as substrates.
ATPase activity of MtbMfd and its mutants were assayed as described previously [38] with minor modifications in 10 µl of buffer containing 40 mM HEPES pH 8.0, 50 mM KCl, 5 mM DTT, 8 mM MgCl2 and 2 mM ATP, 100 µg of bovine serum albumin per ml, 4% glycerol and 6% polyethylene glycol 6000.
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Secretion of the heterologous Kluyveromyces lactis β-galactosidase into culture medium by several Saccharomyces cerevisiae osmotic-remedial thermosensitive-autolytic mutants was assayed and proved that new metabolic abilities were conferred since the constructed strains were able to grow in lactose-containing media.
The hydrolysis activity of Endo-A and the mutants was assayed using ribonuclease B (Sigma-Aldrich, USA) as the substrate.
The effect of SDS on the conformational stability of R-BSX mutants was assayed by comparing the migration of heated and unheated protein samples on SDS-PAGE (Figure 4C & 4D).
The effect of Hha on the cell growth of the prophage mutants was assayed by inducing Hha expression from pCA24N-hha with 1 mM IPTG in LB at 37°C.
However, when the 221/HLA-G mutants are assayed with KIR2DL1+ NK clones, only the triple mutant which mimics the binding site of the HLA-C2 allotypes (i.e. 221/HLA-G M76V+Q79R+T80K) inhibits the killing, in a similar manner to that observed with 221/HLA-Cw4 221/HLA-Cw4-Cw6 (Fig. 4B, dand bars).
More suggestions(15)
variants were assayed
mutants were kept
mutants were confirmed
mutants were obtained
mutants were recovered
mutants were validated
mutants were discovered
mutants were set
mutants were generated
mutants were affected
mutants were indicated
mutants were named
mutants were expressed
mutants were designed
mutants were screened
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