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To investigate the contribution of NcGRA17 α-helices to aberrant morphology of PVs, we transfected four truncated forms of NcGRA17 into NcGRA17 knockout strain and the phenotypes of these mutants were analysed.
To investigate the effect of ethylene signalling on vascular tissue patterning in the hyd/fk mutants, hyd1 ein2 and fkhyd2 ein2 double mutants were analysed.
To examine the effect of charge neutralization on AChE586-599 formationrmation, we used increasing salt concentrations (NaCl and KCl) in a ThT assay, and the effects on both the E2/A and K14/A mutants were analysed.
All mutants were analysed at least three times.
Both the Q63ER and F64AR mutants were analysed for the GAP-stimulated GTPase reaction using HPLC.
The 39 synergistic double mutants were analysed in more detail (Additional file 1: Table S9).
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The most recent transcriptomic analysis of the nodulation programme in M. truncatula [28] has identified five activation profiles from a time course study of nodule development, with symbiotic mutants being analysed independently.
AtMYB60 expression in different cdf mutants was analysed using primers qRT-MYB60-F and qRT-MYB60-R.
Circadian behaviour in the gun1-7, gun1-9 and gun1-9 gun5-1 mutants was analysed with the CAB2 LUC reporter (conferring resistance to kanamycin) that these mutants carry (Koussevitzky et al, 2007).
Because a reduction of cell-cell adhesion is frequently observed in migrating epithelial cells, the influence of the Rac1 mutants was analysed on cellular motility.
To investigate the cell-cycle distribution, the DNA content of ZHBTc4 ES cells expressing Oct-4 deletion mutants was analysed by PI staining and flow cytometry.
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shifts were analysed
ones were analysed
mutants was analysed
mutants were cultivated
mutants were described
mutants were confirmed
mutants were detected
mutants were recovered
mutants were obtained
mutants were discovered
mutants were set
mutants were generated
mutants were monitored
mutants were named
mutants were affected
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