Sentence examples for mutants were amplified from inspiring English sources

Briefly, STING mutants were amplified by PCR or overlap PCR from HA-STING construct, and inserted into pCMV-HA vector with a HA tag at the N-terminus of each STING mutant.

The full-length coding sequence and eIF3f mutants were amplified by PCR to introduce BamH1 and EcoR1 sites on each side of the open reading frame and cloned as BamH1 and EcoR1 fragments into pGEX-3X.

CYLD truncation mutants were amplified by PCR and cloned into CMV promoter-driven pCS2 construct.

All the mutants were amplified from a plasmid after Quickchange mutagenesis using the Expand TAQ polymerase (Roche).

The corresponding regions of the mutation sites of the ltsA mutants were amplified by PCR and the PCR products were cloned into E. coli vector pHSG398.

PLCζWT and mutants were amplified by PCR from the corresponding pCR3 plasmid using Phusion polymerase (Finnzymes) to incorporate a 5′ EcoRI site and a 3′ SalI site and were cloned into pGEX-6P1 (GE Healthcare).

The capB gene from these mutants was amplified by PCR, confirming they were F. tularensis (data not shown).

To control for the introduction of PCR errors, each pool of mutants was amplified in two separate reactions (i.e., two technical replicates per DNA pool).

The DNA fragments encoding the mature region of SaAraf43A and the catalytic domain of SaAraf43A (the CBM42-deleted mutant) were amplified by PCR and then cloned into the expression vector pET30, respectively.

The mouse miR-9-2 gene promoter sequence and its E-box motif mutant were amplified by PCR and cloned into the pGL3 fly-luciferase construct (Promega, Madison, WI), respectively.

To validate the candidate gene models, full-length genomic DNA of each candidate gene in Hwacheong and the sug-h mutant was amplified by PCR.