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However, none of the remaining mutants were altered in their ability to growth in YPD media (Figure 1B).
The vast majority of mutants were altered in their WD repeat domain involved in recognition specificity.
We next determined whether the kinase domain or NPHP-disease mutants were altered in their ability to localize to the nucleus.
Since chloroplasts of both mutants were altered with respect to their size and shape, we examined their effects on the morphology of accumulated starch granules.
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These four Gap1 mutants are altered in a 16-amino-acid span of the NT (positions 20 to 35), close to lysines 9 and 16 (Table 1).
To further prove the role of Porin in mitochondria, we examined whether elongated mitochondria in porin null mutants are altered by increasing the level of mitochondrial fission genes.
In contrast to the uniform staining in the wild-type yeast cells (Fig. 6Ab), some of the ssn8 mutant cells exhibited irregular patches by Eosin Y staining, suggesting the cell wall structure of the ssn8 mutants was altered (Fig. 6Ad and Ae).
GAs content in the ovaries of these three mutants is altered even before pollination and seems to play a key role in the parthenocarpic phenotype [ 8, 20, 21].
mRNA levels corresponding to selected genes which are either up- or down-regulated in dAda2b mutants are altered by dADA2bS expression to different extents, ranging from complete restoration to wild type levels to no restoration at all.
Indeed, tetraspanin null mutants are altered in diverse unrelated processes such as sperm-egg fusion, susceptibility to parasites or viruses, neuronal or lymphocyte cell-cell interactions, cell adhesion, motility and polarity and protein trafficking [ 1, 6, 8].
However, although loss of flight might be a contributing factor it is unlikely to be causative because subsequent data show that lipid metabolism in itpr mutants is altered, and this appears in part to be due to reduced levels of the insulin-like peptide DILP2 (see later).
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