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To confirm the important roles of mtl-1 and mtl-2 genes in regulating the adaptive response to neurobehavioral toxicity observed in mtl-1 tm1770) mtl-1 tm1770125) mutands, mtl-2 gk125 mtl-2 gk125escue experimutantsn nematodes.
To further evaluate the phenotypic consequences of mitochondrial abnormalities in ref(2 P mutants, we next analysed their overall survival.
To further confirm the decreased transcriptional activity of p53 in PTEN mutants, we next did a quantitative analysis of p53-targeted gene expression by real-time quantitative RT PCR (qRT PCR).
Because FIL expression was dramatically reduced in both mp-S319 and brm-3 syd-5 mutants, we next wished to test whether FIL plays a role in flower initiation.
Since no deficit was observed in the central projections of RA mechanoreceptors in E13.5 Gfra1 mutants, we next asked whether cis RET signaling is required for the initial growth of RA mechanosensory third order central projections.
To further evaluate if indeed reduction of ATP directly accounts for bradycardia in ste−/− mutants, we next pharmacologically inhibited ATP production by incubation with 2,4-dinitrophenol (DNP) [ 8, 9, 46].
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Since our imaging and biochemical results indicated that IFT complex B still forms in the dyf13 mutant, we next asked whether DYF13 is required for the movement of IFT particles.
Given the in vitro restoration of genotoxicity displayed by the complemented clbA mutant, we next re-assessed colitis development in CD45RBhigh T cells injected SCID mice fed with Nissle WT, Nissle ∆clbA or Nissle ∆clbA+ clbA.
> Having identified that the expression levels of enzymes involved in lipid metabolism are significantly changed in the Mwk mutant, we next investigated whether levels of lipids were changed as a consequence in the Mwk cerebellum.
Because the TL-5 Gm08-SA intactact (due to contiguity between the Gm08-SA probe hybridization and the Gm08 centromere), and also that a large proportion of Gm08-LA is translocated in the mutant, we next determined the identity of the Gm08 translocation partner.
To analyze cellular mechanisms inducing mutant phenotypes, we next performed cell proliferation and death analyses in the E9.5-TM-treated β-catenin LOF and GOF mutants.
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