Sentence examples for mutants we integrated from inspiring English sources
To determine whether translation of Tfc6p was affected in tfc6 promoter mutants, we integrated nine copies of the myc epitope tag coding sequence onto the 3′-end of the TFC6 gene in wild type and tfc6 mutants to create carboxy-terminal 9 × -myc epitope tagged strains.
To test the drug sensitivity of the HIRAN mutants, we integrated them without GFP under the endogenous rad8 + promoter at the native locus using the Cre recombinase mediated cassette exchange (RMCE) system (see Materials and Methods) (Watson et al. 2008).
In order for ECF to simulate the flux distribution in the central metabolic pathways of the pykF knockout mutant, we integrated the enzyme activity data (Table 2) into the EMC vectors for wild type according to the power law formalism of Eqs.
We integrated the mutants at the endogenous locus without the GFP tag and under the natural promoter.
We integrated this mutant into yeast under the control of the Gal1 promoter and repeated the experiment shown in Figure 4.
Therefore, we integrated a pTET-UME6 construct into the ADH1 locus of the eed1Δ mutant and analyzed the phenotype of eed1Δ after forced expression of UME6.
We integrated the cement plant 10 days ago.
To facilitate genetic analyses and to eliminate the possibility of artifacts due to changes in gene copy number of histone genes expressed from plasmids, for these and subsequent experiments we generated strains in which the genes expressing the histone mutants are integrated into the genome of a common host strain at their endogenous locations (see Materials and Methods and Table 1).
All four shs1-ps mutants were integrated at the endogenous SHS1 locus.
The shs1-ps mutants were integrated into the genome at the SHS1 locus as previously described [60].
