Sentence examples for mutants we generated from inspiring English sources

To evaluate the expression and specific activity of mutants, we generated single-point mutations in the residues that differ from the sequence of the wild type in the mutants M1 to M6 using the PCR mutagenesis strategy (Kirsch and Joly 1998).

To investigate whether the observed dendritic defects were to the result of affected deneddylation in CSN5 mutants, we generated MARCM clones of strong Nedd8 mutations.

Finally, we utilized a series of CXCL10 mutants we generated to map the CXCR3 and GAG binding sites of CXCL10 [28].

To examine the stabilities of TFAM mutants, we generated stable cell lines expressing TFAM, TFAMT56A, or TFAMT56D.

In addition to insertion mutants, we generated three site-specific mutants based on prior studies of dnaQ.

Because of the postnatal lethality of Rfx6−/− and Rfx6ΔEndo mutants, we generated Rfx6fl/fl; Ins1-CreERT2 mice (called hereafter Rfx6Δbeta) to determine the role of Rfx6 in adult β cells.

In order to identify some potential stress and/or compensatory responses in the sgn3 mutant, we generated a general transcriptional profile of sgn3 mutant rosettes, grown under conditions in which wild type and sgn3 were indistinguishable.

To confirm the function of OsBEIIa in the sug-h mutant, we generated dsRNA-mediated interference (RNAi) transgenic plants.

To investigate LHP1 binding in the lif2 mutant, we generated a genomic LHP1 MYC construct; this genomic fragment had been previously used for lhp1 complementation [26].

In order to improve vaccine efficacy and safety of the ΔsecA2 mutant, we generated multiple double and triple mutants of the ΔsecA2 mutant strain via incorporation of other attenuating mutations of M. tuberculosis.

To test for dominant effects in the DFGx mutant, we generated heterozygous IME2/ime2-DFGx strains.