Sentence examples for mutants we extracted from inspiring English sources

To identify the homozygous z3 2 and z3 3 mutants, we extracted genomic DNA from the segregating T1 populations using a cetyl trimethyl ammonium bromide (CTAB) method (Murray and Thompson 1980) and performed PCR analysis.

For analyzing differential expression between the dxr mutant and the WT, we extracted total RNA from 7-day-old seedlings of both genotypes grown under greenhouse conditions.

To check the variance from the effect of RNA-Seq technology on the mutant and wild-type samples, we extracted another set of duplicate samples on Day 12, and the same process was carried out as the previous experiment group.

To assess RubisCO protein expression in the H. neapolitanus mutants, we probed crude extracts of cells that were grown under elevated CO2 for the presence of CbbL protein, using a commercially available antibody that is specific for the conserved large subunit of Form I enzymes.

Total RNAs of S. mobaraensis ECU7480 and its mutants were extracted after cultivation for different times.

The plasmids in the 74 mutants were extracted and transformed into fresh BL21 DE3) cells for a double-check (Fig. 4C).

LPS from R mutants was extracted with phenol-chloroform-light petroleum (2∶5∶8) (165 mg of freeze-dried bacteria/ml) [65].

Total RNA from the testes of P44 mice (5 wild-type and 7 mutants) was extracted with the Trizol reagent.

Metabolite enrichment analysis using other mto mutants also extracted the same pathways [ 31].

Genomic DNA from C. glutamicum transposon mutants was extracted using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich, Taufkirchen, Germany).

Then, plasmid DNA for the 19 most active mutants was extracted and retransformed into naïve yeast for detailed analysis.