Your English writing platform
Discover LudwigExact(15)
In CSN5-null mutants, we did not detect the "intermediate" phenotype.
In particular, in cul1 and cul3 mutants we did not detect disproportional changes in branch distribution (Figure 5D), suggesting that cul1 and cul3 act independently of distance from the cell body.
To maximize curation consistency among curators and to meet the needs of the larger purpose of our work, which is to integrate the phenotypic data of evolutionary morphology with phenotype descriptions of zebrafish mutants, we did a 'first pass' curation of the characters to a coarse level of granularity.
After analysing all of the mutants, we did not find any hot spot mutation regions.
Intriguingly, unlike in previously characterized auxin-response mutants, we did not detect increased AXR3NT-GUS activity in ibr5.
As the purpose of the screen was to identify separation-of-function mutants, we did not further characterize many of the mutants from the screen.
Similar(45)
Classes of mutants we do not expect to isolate are those losing cis-acting sites (such as the origin of replication) and dominant negative mutants.
Although we found recombinant MtrA-His6 is more stable in proteasome-defective mutants, we do not know if endogenous MtrA accumulates in mpa or pafA mutants, or if pupylation affects MtrA activity.
Although we do not have a structure of the non-phosphorylated form of KaiC at this time (or of the KaiCTaa, KaiCaaa, and KaiCaST mutants), we do not expect the conformations of the hypo- and hyper-phosphorylated forms to be drastically different.
Thus, it is not surprising that in cases where selection is strong, such as in the case of resistance or escape mutants, we don't see quasispecies effects.
However when we triggered the cells with the ΔphoP mutant, we did not observe ROS production or pH changes.
More suggestions(15)
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com