Sentence examples for mutants we created from inspiring English sources

Due to difficulties with making either rsc1Δrco1Δ, or rsc2Δrco1Δ double mutants, we created an Rco1-degron strain (Rco1-deg), using the system described in Kanemaki et al. [52].

To test if loss of tsp66E could rescue the SJ membrane accumulation observed in CrebA mutants, we created embryos homozygous for null mutations in both genes and examined the SG lateral membranes.

To better understand the relevance of the genetic interactions in the three different DNA polymerase mutants, we created Profilyzer, an interactive, fitness-profiling web-tool (http://research.ncl.ac.uk/qfa/Dubarry2015/).ac.uk/qfa/Dubarry2015/

Building on previous studies of DsRed mutants, we created two DsRed-Express2 derivatives: E2-Orange, an orange FP, and E2-Red/Green, a dual-color FP with both red and green emission.

Instead of generating a targeted deletion mutant, we created six serial deletions (each was ∼100 bp shorter than the adjacent one) by PCR amplification using pGL/WTH3P as the template.

To determine if the lariat precursor remains the major pathway for circular RNA biogenesis in this mutant, we created an mrps16 mutant containing both deletion of the upstream 5′ ss and downstream branch point (to halt production of the lariat precursor), and, indeed, circular RNA production is strongly abated.

The allele designated cyp85A2-2 in Kim et al., 2005 is a different allele (salk_068754) that was designated cyp85A2-3 by Nomura et al. To further characterize the seu cyp85A2 double mutant phenotype we created and analyzed the following double mutants: seu-3 cyp85A2-1, seu-3 cyp85A2-2 (both in the Col-0 background) and seu-1 cyp85A2-4 (L er background).

To further increase complementarity to mutant 5′ss, we created U1snRNAs specific for each mutation (U1F9spec; Fig.  3A).

As both melanophores and iridophores are affected in tra mutant adults, we created chimeric animals to address in which cell type tra is required.

However, instead of increasing growth and methane production, the att:: hdrABC* mutant strain we created had a growth rate identical to that of the parent strain (Fig. 2A).

Next, to examine the inhibitory activity of CysC treatment on CatB activation by mutant SOD1 expression, we created a W106G CysC mutant that specifically lacked the inhibitory activity against CatB.