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We therefore detected FBXO7 in insoluble fractions, but the amount of FBXO7 mutants was still lower than that of the WT counterpart (data not shown).
In the present study, we found that all of these mutants only partially abrogated the repressive function of CBX7 on p16 expression, because the residual function of these mutants was still significant.
Mutation of Ser88 and Leu90 markedly reduced basal MST3 activity but the activity of these mutants was still enhanced 2- to 3-fold by MO25α (Fig. 4B).
Remyelination in conNrg1 mutants was still deficient at this stage, and in particular a significant proportion of large axons were ensheathed by Schwann cells but had failed to elaborate a myelin sheath (Fig. 4C and G).
When K170, K194 and H198 residues were mutated in combination with each other, unwinding activity by the double mutants was still greater than the WT and when all three were replaced with alanines, unwinding activity returned to the WT level.
At the same time, AZ localization of both BRP and RBP at synaptic terminals of aplip1 mutants was still observed in both aplip1 alleles (not shown), although slightly reduced (not shown).
Similar(53)
We hypothesized that the KNtp of these mutants are still able to bind SUR1 but the restriction of the CTD rotation by KNtp might be reduced.
What's more, the authors report, the mutants were still drinking substantially more than the controls 6 months after their unpleasant involuntary swims, the team reports in the 3 May issue of Science.
Unfortunately, some of the mutants were still insoluble in the E. coli expression system and were excluded from further studies.
Third, the ssn8 mutants were still responsive to light and their mating filaments were inhibited by light (data not shown).
The results showed that the RT mutants were still undetectable in the presence of EFV (Fig. 5A).
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CEO of Professional Science Editing for Scientists @ prosciediting.com