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Firstly, the migration phenotype of fra mutants was slightly enhanced by loss of netrins.
Surprisingly, qPCR assays demonstrated that the relative level of disc1 expression in pools of ptch1 ptch2 mutants was slightly reduced compared with sibling controls (Fig. 3B).
Comparison of the growth curves of wild-type P. gingivalis strain 33277 and the ECF mutants indicated that the growth rate of the mutants was slightly lower than that of the wild-type strain.
Interestingly, on MS medium without stress treatment, root length of luh-4 and slk1-1 mutants was slightly shorter compared to wild type plants due to slower root growth.
Leucaena and siratro also did not show differences in nodulation between the WT and the three mutants, but SDW of leucaena inoculated with nodD4 and nodD5 mutants was slightly, but significantly, lower (Table 1).
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In our studies, we confirmed previously reported phenotypes, namely, the cpd and ga1 mutants being slightly late flowering, and the aba2 mutant exhibiting modest early flowering.
The basal levels of ATL9 in each of the mutants are slightly lower or higher than in Col-0 (inset Figure 2A).
The mutants were slightly less sensitive than WT to 3-O-methylglucose which weakly induced anthocyanin accumulation (Figure 1F and [40]).
The newly analyzed intermediate mutants T215S, T215N, M41L-T215S and M41L-T215N of the AZT-resistance pathway (Figure 1) exhibit low fitness values under all drug concentrations with the 215S mutants being slightly fitter than the 215N.
Although both the daf-2 e1368) daf-2 e1368utands we1370lightly longer than wildtype animutantsder non-DR conditions, these differences were not aslightlyic as for DR conditions, suggesting that DR enhanced the growth-promoting effect of daf-2 mutations [33].
Both xprG1 and xprG - mutants were slightly slower to initiate conidiophore development.
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