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The glpT gene of all mutants was sequenced, and compared with the wild type gene as described [37].
In addition, a library of 100 random mutants was sequenced to determine our ability to detect unique mutations.
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In, addition, 61 and 54 mutants were sequenced in control and exposed groups, respectively.
Randomly selected transposon carrying protease negative mutants were sequenced and alignment of these sequences lead to the identification of the protease open reading frame (ORF).
The mutants were sequenced, and we verified by western blotting that the proteins expressed had the expected size.
The TBCD mutants were sequenced and cloned into the pEGFP-C2 and/or pEGFP-N3 vectors from Invitrogen (Life technologies, California USA).
All mutants were sequenced to verify the intended mutation.
Validated mutants were sequenced and sequences were analyzed with Chromas and DNASTAR softwares.
All mutants were sequenced, and the clones were verified for the mutation.
The protein coding region of all chimeras and mutants were sequenced.
Finally, the mutants were sequenced and inserted into the pGL3 luciferase vector as described above.
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