The list of FST genes with related gene-indexed mutants is provided in Additional file 1: Table S1.
GFP-tagged full-length BRCA1 and BRCA1 deletion mutants were provided by Dr. N. Chiba [68].
A list of primers used to generate and confirm the allelic replacement mutants is provided (Table 3).
Full details on fatty acid profiles of these mutants are provided in Table 1.
All experimental details of expression profiling the deletion mutants are provided in Additional file 3.
Details for the generation of the H3-L61X mutares are provided below.
The sequences of piRNAs and their corresponding mutants are provided in Supplementary information, Figures S5B and S9A.
An overview of the results of the KEGG pathway enrichment analyses of the two mutants is provided in Additional file 1: Table S6.
The experimental procedures used for the construction of the P1A, R70A, R73A, and E114Q Cg10062 mutants are provided in the Supporting Information.
The DGRP collection of flies was obtained from the Bloomington Stock Center, the African collection was provided by John Pool (UW-Madison), and grh mutants were provided by Melissa Harrison (UW-Madison) (Mackay et al., 2012; Bastide et al., 2014).
Analysis of lin-28 gfp lin-28 gfpched" wt and ced-3(lf) mutants in provided through a picture of a head together wtth quanditation that indiced-3 lfmodest ced-3 lf in numutants GFP+ cells when ced-3 is missing.
