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The frequency of occurrence of rifampicin resistance mutants was obtained by plating bacteria onto GCB agar plates supplemented or not with 30 mg/mL of rifampicin.
The CD spectrum of purified His6-HbpR or of its mutants was obtained from a J810 spectropolarimeter (Jasco, Tokyo, Japan) using a quartz cell with a 0.1-cm path length (L).
No PLP synthesis activity of the plasmodial Pdx1 mutants was obtained; however, the glutaminase activity of PfPdx2 remained, although it was reduced to approx. 66% and 36% in the presence of the PfPdx1 mutants H88A and E91A, respectively (Table 1).
No data on SopE mutants was obtained for the mouse infection model so the importance of SopE in mammalian infection or gut colonization is unclear.
A total of 150 CAD and C3H mutants was obtained by screening for mutations with the mismatch-specific endonuclease ENDO1 as previously described [ 37].
A lineage of increasingly polymyxin B-resistant heptoseless LPS mutants was obtained through selection of resistant isolates on media that prohibited the replication of the vast majority of the cells plated.
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By following this protocol, 335 Sav mutants from the targeted 336 Sav mutants are obtained.
The wt hAID enzyme and selected mutants were obtained in a bacterial expression system (see Methods).
SxVis (ExoS233 409 fused to Vis218 249) and the ExoS233 453 mutants were obtained as synthetic clones, sub-cloned in pET151b (GeneArt/ThermoFischer Scientific).
Through site-specific mutagenesis, several improved mutants were obtained, with GadB1E312S being the best one.
Via this method, four new fluorescent protein mutants were obtained based on monomeric cherry fluorescent protein.
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ones was obtained
transformants was obtained
mutants was verified
mutants was complemented
mutants was evaluated
mutants was screened
mutants were obtained
mutants was named
mutants was used
mutants was generated
mutants was repeated
mutants was prepared
mutants was accompanied
mutants was performed
mutants was confirmed
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