Sentence examples for mutants was isolated from inspiring English sources
To exclude the isolation of bacteriocin-tolerant contaminant bacteria, genomic DNA of the presumed mutants was isolated and the boxA element was amplified using primer BOXA1R (PGPRB-10181; supplementary table S1, Supplementary Material online) as described (Koeuth et al. 1995).
In a first evolution round employing random mutagenesis, which included about 106 mutants, a set of interesting mutants was isolated.
Total RNA from the Huh7 cells transfected with full-length HBx or HBx mutants was isolated using the Trizol reagent, according to the manufacturer's instructions (Invitrogen Life Technologies).
In sorghum (Sorghum bicolor), a series of brown midrib (bmr) mutants was isolated from diethyl sulfate (DES) mutagenized populations at Purdue University in the 1970s (Porter et al. 1978; Bittinger et al. 1981).
Each of these mutants was isolated repetitively from many distinct mice treated with distinct M2e-specific MAbs, which indicates that they represented essentially the entire range of escape mutants capable of arising from the PR8 wild-type virus under the given experimental conditions.
Briefly, genomic DNA from 175 in vitro isolated PZA-resistant mutants was isolated (see below) and used as templates for PCR as follows: heat denaturation at 94 °C 15 min followed by 30 cycles of 94 °C 0.5 min, 55 °C 0.5 min, 72 °C 1 min followed by extension at 72 °C for 7 min.
Induced Sal-resistant mutants were isolated using procedures analogous to those used for isolation of induced Myx-resistant mutants in Mukhopadhyay et al. (2008).
Human SNAP-23 cleaving mutants were isolated using a newly established yeast-based screening system.
The plasmids from the mutants were isolated and the regions adjacent to the transposons were sequenced using transposon specific primer.
Over 100 mutants were isolated at a frequency up to ninefold higher than that of uninfected controls.
In rhizobia, desiccation sensitive mutants were isolated with mutations in the DNA repair systems (Humann et al. 2009), confirming that rhizobia are similarly affected by desiccation.
