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The lifespan of tax-4 p678 tax-4 p6781426) rrf-3 pk1426ts rrf-3 pk1426double these animutantsre fed wash smg-1 RNAincreased1), suggesting that tax-4 and smg-1 act independently to regulate lifespan.
Although growth rate of the mutants was less affected than wild type, the peak luminescence for two of the mutants was increased in the presence of Vp cell-free culture fluids whereas wild type luminescence was not increased (Table 5; Figure 6B).
Moreover, the Gal-1-dose-dependent RBD recruitment response of both mutants was increased in cells, while RBD-binding in vitro remained indistinguishable from the parent Ras.
The finding that the level of O-GlcNAc signals in the chromatin from Oga mutants was increased suggests an active cycling of the modification at target genes in wild-type animals (Love et al. 2010).
With the exception of the bub1 cells, at day 7 of chronological culture, the RLS of other mutants was increased compared to day 2, while at day 14 RLS was moderately decreased (the bub2 and mad1 cells) or increased (the tel1 cells) compared to day 2 (Fig. 2).
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The Km values of mutants were increased compared to native form, indicating lower affinity of mutants toward substrates.
The organic solvent tolerance levels of these mutants were increased in the following order: JA300 < JA300 acrR IS < JA300 marR < JA300 acrR IS marR.
In these mutations, the Km values are decreased and the kcat values are decreased, meaning that the affinities of the mutants are increased but the rates of the catalysis are decreased.
It has been shown that DnaK mRNA and DnaK protein of Streptococcus mutants were increased in response to acid shock (Jayaraman et al. 1997), and the strain's capacity for growth in low-pH media becomes impaired, when DnaK levels of S. mutans are lowered via knockdown, strongly indicating the involvement of DnaK in acid tolerance (Lemos et al. 2007).
In our conditions, the period of defecation in both clk-1 and clk-3 mutants is increased by about 40%.
When compared with Pomc −/− mice, obesity in Pomc −/−Tg + mutants is increased and the rate of weight gain is more rapid.
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