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Localization of polarity markers in heterozygous mutants was in all cases comparable to wild type (not shown).
The lethality of Dll knock-out mutants was in accordance with previous studies on insects [ 25, 32].
The lack of a phenotype in neural-specific Kdr mutants was in striking contrast to the reduced number of GnRH neurons in mutants lacking NRP1 in the neural lineage (see above, Fig. 2G,H).
Meanwhile, the value of absorbance of different bacterial mutants was in sequence of group A3 > G6 > D1, which suggested that bacterial flora processed by mutation group D1 had the best total protein activity.
These results demonstrate that the aphidicolin-sensitive phenotype of osrecql4 mutants was in part due to induced DSBs and cell death, and that OsRecQl4 plays an important role as a caretaker, maintaining genome stability during DNA replication stress in the rice meristem.
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The kcat values of the mutants were in the range of 47.2-110 47.2-110s−1values were 52-120% of theseild-type enzyme values
These mutants are in the Arabidopsis thaliana Columbia accession (Col).
All mutants are in the Arabidopsis Columbia ecotypic background (Col-0).
The mutants are in two classes, within dimers and between dimers.
All mutants were in the Col-0 background except for hen1-1 whish in in the La-er background.
Six of the missense mutants are in EGF modules and two are in the DPY members of the diads.
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