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The collection of single and double bat1Δ and bat2Δ mutants was grown on ethanol as carbon source and ammonium as nitrogen source.
One of the mutants was grown outdoors in commercial-sized open ponds of 125 000 L in the north of Chile.
Each strain (wild type and mutants) was grown in 50-mL flasks with 30 mL of optimal Haematococcus medium (Fabregas et al. 2000).
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Congenic wild-type and pri2 mutants were grown at 23°C to log phase.
(c) Col-0 and AtBS14b mutants were grown under continuous dim light for 7 days and seedlings were photographed.
a WT and the indicated mutants were grown on solid THY (sucrose+) plates with for 24 h, and the colonies were photographed.
a WT and the indicated mutants were grown on solid THY (sucrose+) plates for 24 h, and the colonies were photographed.
(B) Congenic wild-type and pri2 mutants were grown at 23°C to log phase and then shifted to 37°C for an additional 2, 4 and 8 h.
The cpk1 and ssn8 single mutants, and three cpk1ssn8 double mutants were grown in YPD liquid medium.
C. neoformans MATα strains including three ssn8 mutants were grown in YPD liquid medium for 22 hours.
The wild-type strain and the three mutants were grown 36 hours at 37°C on BUG agar (Biolog).
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