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Moreover, overexpression of dominant negative CKB mutants was found to promote an epithelial-to-mesenchymal transition (EMT) in colon cancer cells [45].
Although the majority were TS for function in vivo, all of the mutant enzymes were stably expressed and largely soluble at both 30 degrees C and 42 degrees C in vivo and none of the mutants was found to be TS in vitro.
The NCBI collection of avian sequences had 2 out of 5991 (0.03%) mutants with more than a single mutation, while in the Ottenby collection the same type of mutants was found in 4 out of 230 (1.74%) isolates.
A majority (>90%) of the recombinant protein and its mutants was found to be in the soluble fraction, and showed comparable biological activity with the native protein from Bacillus.
An allelic pair of newly described mutants was found.
The association between the KRAS mutant genotype with SMAD4 mutants was found to be significant (P =< 0.05).
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These mutants were found to be cross resistant to FTC.
In addition, a few gene product alterations specific for each of the multi-step mutants were found.
Five tall mutants were found in DongnongV7 variety, and the average height of the mutants was 31% taller than that of the control.
Rather, multiple W-to-G substitutions were required to inactivate Cef1p, and many of the substitution mutants were found to confer temperature sensitivity.
The mutants were found to be less than half as responsive as their wild-type conspecifics to the highest modafinil dose (40 mg/kg) (Stone et al, 2002a).
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