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The library of CYP102A2 mutants was expressed in BL21 DE3) Escherichia coli cells and screened for their ability to oxidize different substrates by means of an activity assay.
The odd bubble-like morphology of the TC/LT region seen in Ptp4E Ptp10D, Btl>Rho1-CA embryos was not obviously changed when any of the DN RTK mutants was expressed (Fig. 8F H).
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RAM GFP and PY mutants were expressed in HeLa cells resulting in equivalent expression.
The wild-type cueO gene and mutants were expressed in E. coli BL21 (DE3) using the pET expression system.
Twenty-three double mutants and twenty-two single mutants were expressed by Pichia pastoris.
These mutants were expressed as recombinant, codon-optimized proteins in E. coli.
The mutants were expressed in E. coli and purified to homogeneity.
The DraIII mutants were expressed and purified using the same methods as for wild type (WT).
All 6 mutants were expressed in Escherichia coli (BL21) and purified.
Some of the resulting mutants were expressed in the soluble form.
The NDK from Leishmania major (LmNDK) and mutants were expressed and purified to homogeneity.
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