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Immunoblot analysis of the reconstituted MEFs verified that expression of the Stat1 mutants was equal to that of Stat1 WT (Fig. 2A, panel a).
As a control, we demonstrated that the membrane expression of the dimerization motif and dimerization pocket mutants was equal to that of the wild-type and kinase-inactive TRPM6, suggesting that these mutations do not hamper expression and transport to the plasma membrane.
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Sabapathy, K. & Lane, D. P. Therapeutic targeting of p53: all mutants are equal, but some mutants are more equal than others.
Although the charge state distributions between mutants were equal, S32 variants were found to possess higher abundances of charge-states from +9 to +21 relative to their W32 counterparts, suggesting a greater proportion of unfolded SOD1 after reduction and metal chelation (Figs 1E and S1).
The expression level of WT and mutants are equal.
The results demonstrate that no rough mutant is equal to Rev 1 in laboratory models and question the notion that rough vaccines are suitable for the control of brucellosis in endemic areas.
The skeletal muscle cross-sectional area (CSA) of 3-dpf-old siblings was 0.0328±0.0005 mm, and that of tmod4 trg mutants was 0.0309±0.0004 mm (equalling a reduction to 94±1%, P<0.01, n=4).
For transfections involving MALT1-API2, a double amount of DNA of the wild-type MALT1-API2 variants compared to their RING mutants was used to obtain more equal expression levels.
The expression of all mutants was almost equal to that of ND-HBD* (Supplementary information, Figure S4C).
Consistent with measurements at 3 dpf, the muscle of tmod4 trg mutants was only very slightly reduced in size; the CSA of sibling muscle was 0.0324±0.0007 mm and that of tmod4 trg mutants was 0.0315±0.0004 mm (equalling a reduction to 97±1%, P<0.01, n=4).
ac-tRNA delivery to IRES 80S ribosome complexes with the △1 and △2 mutants was less than to WT, but equal to or greater than to the IRES with a non-cognate codon.
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