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To determine if reduced Brp density and loss of Syb in wnt2 mutants was due to changes in gene transcription, we examined brp and syb mRNA levels in 1st, 2nd, and 3rd instar larvae using quantitative RT-PCR.
To test whether the increased resistance observed in glp-4 and glp-1 mutants was due to a lack of matricide, we compared the survival of N2 wild-type, glp-4 mutant, and glp-1 mutant nematodes to that of fer-1 and fer-15 mutant nematodes.
To exclude the possibility that the conditioning defect in magi-1 lf) magi-1 lfas due to changes in adaptation rather than association, we conditioned mutantslf) mutants and wasdueype contools with DA in the presenchangesbundant food (Fin. 1b).
However, additional studies demonstrated that the dissemination of S. enterica throughout the different tissues of the mutants was due to the internal hatching of eggs, which disrupts tissues causing matricide, rather than to specific deficiencies in responses that prevent S. enterica invasion such as those mediated by TOL-1 [18].
To determine if the decreased number of GABAergic synapses in ju89 mutants was due solely to defects in initial axon outgrowth and extension along the nerve cords, we examined the morphology of the dorsal D (DD) motor neurons in more detail.
Thus, the abnormal PGC morphology of these mutants was due to reduced Neurl4 activity.
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Previous studies suggested that PLA1 and PLA2 genes regulate the rate of leaf maturation (Kawakatsu et al.[2006]) and that the small leaves in pla mutants were due to precocious leaf maturation.
To confirm that the defects observed in lin-23 mutants were due to a lack of BAR-1/β-catenin regulation, we constructed double bar-1; lin-23 mutants.
To unequivocally show that the observed phenotypes of the mutants were due to the lack of Pngl, we investigated the effect of Pngl transgene expression in these mutants.
In a second model, it is possible that the defective ethylene responses of hyd/fk mutants are due to aberrant auxin responses.
To investigate if the increases in cell viability in different c-CBL mutants are due to increased cellular proliferation, a cell cycle analysis was performed.
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