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However, in order to confirm that the low gluconic acid production was not caused by different growth rates of the Δgox mutants, the biomass of the Δgox mutants was determined after the growth experiment.
The feeding ability of itpr mutants was determined quantitatively by measuring ingestion of colored food (Figure 2).
The degree of cytotoxicity among the 28 mutants was determined by measuring the release of lactate dehydrogenase (LDH), a cytoplasmic protein commonly used to identify cell lysis.
The photosynthetic performance of the site-directed mutants was determined by chlorophyll fluorescence analyses, using the Photo II device (Biosensor Srl, Palombara Sabina, RM, Italy, http://www.biosensor.it/).
The bactericidal activity of 20% fresh guinea pig serum or various dilutions of human serum for F. tularensis mutants was determined as previously described [59].
The activity of the wildtype mouse protein (wtmBMPR1A) and its mutants was determined by measuring induced Luciferase activity in the transiently transfected pre-myoblastic mouse cell line C2C12 (ATCC).
Similar(32)
Some of the mutants were determined as superiors for the studied variables.
Binding changes of the individual mutants were determined using three saliva samples that are Lea (Nonsec/Lea), Ley (Sec/Ley) and Leb (Sec/Leb) positive, respectively (Fig. 8B).
The metal contents of the wild-type enzyme and mutants were determined by inductively coupled plasma optical emission spectrometer (ICP-OES), PerkinElmer 5300DV.
The structures of PKM2 mutants were determined by molecular replacement using the monomer of PKM2 wild-type structure (3BJT.pdb) as a searching model (Christofk et al., 2008b).
The nucleotide sequences of entire acrR, marR, and soxR genes in organic solvent-tolerant mutants were determined by DNA sequencing to identify mutations in these genes.
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variants was determined
changes was determined
ones was determined
mutants was verified
mutants was complemented
mutants was found
mutants was enriched
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mutants were determined
mutants was screened
mutants was named
mutants was used
mutants was carried
mutants was described
mutants was accompanied
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