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Expression of the different Eps15 mutants was detected by GFP fluorescence (Figure S3, left column).
Pr160gag-pol in lysates of cells transfected with the wild type and mutants was detected by Western blotting.
PCMT activity in endothelial cells transfected with vectors carrying PCMT sense or antisense or either mutants, was detected by means of the radiochemical assay described by Macfarlane, using ovalbumin as a standard methyl-accepting substrate [41].
When HERG was immunoprecipitated and assayed for co-precipitated KCNE proteins evidence for physical association with each of the KCNE1 and KCNE2 and their mutants was detected (Figure 1A, lower panel).
Bound angiocidin or its mutants was detected with a biotinylated-labelled rabbit antiangiocidin antibody raised against recombinant angiocidin.
RNMT co-purifying with GST RAM and mutants was detected by Coommassie Blue-standed SDS/PAGE and Western blotting.
Similar(48)
The YFP signal levels in the roots of WT, two single mutants, and double mutants were detected.
Mutants are detected using a thymidine analogue that arrests the growth of cells containing a functional Tk gene.
For parasites found in the mosquito midgut, cycloguanil-resistant mutants were at 90percentt, while pyrimethamine-resistant mutants were detected between 2 and 12 percent.
The data showed that no significant differences between wild-type and mutants were detected (Additional file 6: Figure S5).
The mRNA level and protein expression of wild type eIF5B and two mutants were detected by qPCR, Western blotting and mass spectrometry, as shown in Figs. S3, S4 and S5.
More suggestions(15)
genes was detected
transformants was detected
mutants was verified
mutants was complemented
mutants was found
mutants was enriched
mutants was evaluated
mutants were detected
mutants was screened
mutants was named
mutants was carried
mutants was described
mutants was accompanied
mutants was performed
mutants was counted
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