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Relative ligand-binding capacity (represented by binding %) to different mutants was calculated by the MS response of the ligand released from a specific mutant divided by the MS response of the total ligand present in the incubation sample.
The wt TSHR was set at 100% and receptor expression of the mutants was calculated according to this.
The concentration of the mature inhibin A or activin A mutants was calculated based on the immunoblot.
The Cm value for ΔQ2, Q2R, and ΔP3 mutants was calculated to be 21.85, 19.84 and 17.94±0.05 M, respectively.
The level of wild-type TSHR was set as 100% and receptor expression of the mutants was calculated according to the level of wild-type TSHR.
The absolute quantity of xylan in five mutants was calculated by using internal quantitative standards.
Similar(47)
Assuming that the structures obtained in the high temperature simulations (400 K or 450 K, 0.4 1.4 μs) correspond to the denatured ensembles, which are in equilibrium with folded structures under physiological conditions, hydrophobic energies of the three mutants were calculated (Table 3).
RT activities of mutants were calculated and compared to that of wild type pseudovirus.
After incubation at 37°C for 48 h the numbers of CFU were counted, and the frequencies of rifampicin resistant and streptomycin resistant mutants were calculated.
Extinction coefficients of the Cygb distal histidine mutants were calculated as previously described using HPLC.
Structural models of selected cancer-associated single-point mutants were calculated by the SWISS-MODEL server [ 37].
More suggestions(15)
ones was calculated
variants was calculated
mutants was complemented
mutants was verified
mutants was evaluated
mutants was screened
mutants was named
mutants was used
mutants was generated
mutants was repeated
mutants was prepared
mutants was accompanied
mutants was performed
mutants was confirmed
mutants was written
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