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Secretion of the heterologous Kluyveromyces lactis β-galactosidase into culture medium by several Saccharomyces cerevisiae osmotic-remedial thermosensitive-autolytic mutants was assayed and proved that new metabolic abilities were conferred since the constructed strains were able to grow in lactose-containing media.
The hydrolysis activity of Endo-A and the mutants was assayed using ribonuclease B (Sigma-Aldrich, USA) as the substrate.
The effect of Hha on the cell growth of the prophage mutants was assayed by inducing Hha expression from pCA24N-hha with 1 mM IPTG in LB at 37°C.
The effect of SDS on the conformational stability of R-BSX mutants was assayed by comparing the migration of heated and unheated protein samples on SDS-PAGE (Figure 4C & 4D).
(B ) Label transfer from tRNA-ProUGG mutants was assayed with Ssa2p.
Once constructed, the library of mutants was assayed for transcriptional activity on the p21 promoter.
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During the first vinification experiment (V1) the haploid and diploid pdc2Δ519 mutants were assayed against their respective BY4741 and BY4743 controls (Table 1).
The native form and cysteine/serine mutants were assayed for enzyme activity, free thiols, and the secondary structures by circular dichroism and Fourier transform infrared.
The resultant double mutants were assayed for their ability to grow on plates lacking histidine.
dop-3 single mutants were assayed off food for avoidance of dilute (30% and 10%) octanol.
The enzymatic activities of the wild type PDE9A2 and its mutants were assayed by using cAMP and cGMP as substrates.
More suggestions(15)
mutants was investigated
mutants was measured
transformants was assayed
clones was assayed
mutants was ascertained
variants was assayed
mutants was evaluated
mutants was corroborated
mutants was verified
mutants was complemented
mutants was screened
mutants was named
mutants was used
mutants was generated
mutants were assayed
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