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Periplasmic expression level of the mutants was also improved.
Ability to produce nitrate nonutilising (nit) mutants was also assayed.
The generation of escape mutants was also blocked as evaluated by long-term culture of challenged cells.
High expression of the lux systems of Vibrio harveyi, Photobacterium leiognathi, and Xenorhabdus luminescens in E. coli MC4110 hns rpoS cells compared with that in wild-type or rpoS mutants was also accomplished.
A drastic decrease of OTA production that ranged from 68.5 to 99.4% in ΔveA and ΔlaeA null mutants was also observed, which was correlated with a downregulation of a nonribosomal peptide synthetase involved in OTA biosynthesis.
The germination rate of the mutants was also different.
And the memory formation of these svr-GAL4 mutants was also impaired (Fig. S1).
Furthermore, at adult stage, the root gravitropic set angle of docs1 mutants was also affected since docs1 mutant plants displayed larger root cone angles.
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Exemplary mutants were also produced and compared to native protein.
Mutants were also generated in which At4g18010-P3 BRRE and At4g00360-P3 E-box were mutated to unrelated sequences (see Fig. 5a).
This cleavage was not an autocatalytic process since the E385/A mutants were also processed.
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