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Although the saccharification efficiencies were nearly similar, due to the high cellulase activity the required amount of enzyme from mutants was almost threefold lower than the wild-type.
On the other hand, the change in cell length in the double mutants was almost the same as that in the single mutants.
The growth of all the mutants was almost similar in terms of dry cell mass and thus the specific bikaverin production by Mut-4 was also highest reaching 317 mg bikaverin g−1 dry cell weight against just 6.5 mg bikaverin g−1 dry cell weight by the parent.
Another remarkable difference between the two data sets was observed in the N6 subtype, where the proportion of mutants was almost 20 times higher in the Ottenby than the NCBI data set (Table 2).
The expression of all mutants was almost equal to that of ND-HBD* (Supplementary information, Figure S4C).
However, after 20 J/m of UV irradiation, the growth rate of these mutants was almost identical to that of the rad2C65Δ mutant (supplementary material Fig. S3B).
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Since the genomic sequences of host-range phage mutants are almost identical, sequencing PCR amplicons of the most variable regions could distinguish the phages in commercial products from different companies.
However, the changes in deactivation time constant (τdeac) of BK channel caused by WT β4 subunit and its mutants were almost similar (Fig. 5d), consistent to the fact that β4 subunit distinctly slows BK channel deactivation kinetics.
Our genetic data show that the early-flowering phenotype of brm mutants is almost, but not completely reverted in a ft background (Figure 1C), suggesting that FT mostly contributes to the early-flowering phenotype of brm.
Thus, gp150-specific monoclonal antibodies (mAbs) increase MuHV-4 infection of GAG-deficient cells [8] and gp150− mutants are almost completely GAG-independent: they infect GAG+ cells normally, infect GAG− cells much better than the wild-type does, and largely resist inhibition by soluble heparin.
The limb phenotypes of the Wnt5a mutants and the Ror2/Vangl2 double mutants are almost identical [ 17].
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