Sentence examples for mutants utilizing a from inspiring English sources

To clarify further how IMPase regulates the synaptic polarity and consequently sensory behaviors, we conducted a genetic suppressor screen for ttx-7 mutants utilizing a population thermotaxis assay (Materials and Methods).

These F. novicida mutants utilize a highly efficient mechanism for entry into macrophages that does not involve eukaryotic microfilaments.

Finally, we were unable to rescue the ethanol hyperactivity phenotype of DopR mutants utilizing mushroom body GAL4 drivers (see below).

Instead, a proposed direct route to ethylene glycol from D-arabinose that has been demonstrated in E. coli mutants utilizes the L-fucose pathway, a pathway which appears also to be present in C. saccharolyticus[ 18].

Cloning strategies to generate the various mutants utilized in this study were as follows: For pET15b/eIF4AIIG, two PCR products were produced using pET15b/eIF4AI as template and the primer pairs: (i) DraIII Forward and IG Reverse and (ii) IG Forward and BamHI Reverse (Table S1).

We found an increase in glutamate receptor levels due to wild-type TDP-43 expression, which provides further support for the notion that the TDP-43 mutants utilize distinct cellular mechanisms.

To understand how long-lived mutants utilize acetic acid, we treated wild-type and sch9∆ mutant cells with NaCN (0.25 m m), a respiration inhibitor, on day 1 or day 3. Treatment of sch9∆ mutants with NaCN every other day until day 7 delayed the utilization of acetic acid.

The mutant transporters were constructed utilizing a Stratagene Quickchange XL site-directed mutagenesis kit.

Through in vitro transcription and in vivo experiments (utilizing a RapAR599/Q602 mutant lacking transcription-stimulatory function), we demonstrate that the formation of tandem (α2ββ'ω 2 DNA complexes is likely functionally significant and beneficial for the transcriptional activity of the polymerase.

To more directly assess nutrient intake in ATPalpha CJ mutants we utilized an established Drosophila feeding assay (Edgecomb et al. 1994; Xu et al. 2008).

We next determined if it is the alteration in RhoA activity utilizing either dominant negative (dn) mutants, constitutively active (ca) mutants [11] and/or expression utilizing an siRNA against RhoA that underlies stimulated NHE1 activity in transformed cells.