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Lu, B. et al. Generation of rat mutants using a coat color-tagged Sleeping Beauty transposon system.
To confirm this we designed inhibitor-resistant (ir -Cdk2 mutants usir -Cdk2vel bioinformutantsapproach.
The analysis of secondary structure revealed that all mutant enzymes presented structural content slightly different to the wild type enzyme (Fig. 3C; Table 3), similar to the observed to AspG and similar to other mutants using a different approach22.
We produced these chimeric mutants using a similar strategy as that used for producing hTIM-1 and verified them as functional units by thermal stability assays (Fig. S4).
hip3-1 mutant was isolated by screening for suppressor mutants using a reporter plasmid.
Complementation of the mutants using a high-copy overexpression vector failed, while utilization of a low-copy inducible vector successfully restored L-form formation.
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The isolate SAKA10119 showed the best ability to adhere to HCT-8 cells, and it was therefore selected to generate transposon mutants using an EZ-Tn5™ Transposome Kit (Epicenter, Madison, USA).
We further examined the proapoptotic activities of WT and each of various Bax mutants using an ApoAlert Annexin-V kit as described [36] [37].
We sought to test this hypothesis by testing pre-let-7a-1 and its CTL mutants using an in vitro uridylation assay.
cheZ mutants use a weighting that extends at least 40 sec into the past.
As described in detail previously [ 19], the generation of transformed Vac- (minus) halobacterial SD109 mutants used a series of plasmids.
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